JoVE Logo
Faculty Resource Center

Sign In

Imaging Calcium Dynamics in Subpopulations of Mouse Pancreatic Islet Cells

DOI :

10.3791/59491-v

November 26th, 2019

November 26th, 2019

7,584 Views

1Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, 2Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre, Malmö University HospitalMalmö, 3Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit, University of Göteborg, 4Oxford National Institute for Health Research, Biomedical Research Centre, 5Life and Medical Sciences, University of Hertfordshire

Here, we present a protocol for imaging and quantifying calcium dynamics in heterogeneous cell populations, such as pancreatic islet cells. Fluorescent reporters are delivered into the peripheral layer of cells within the islet, which is then immobilized and imaged, and per-cell analysis of the dynamics of fluorescence intensity is performed.

Tags

Calcium Dynamics

-- Views

Related Videos

article

Murine Pancreatic Islet Isolation

article

Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue

article

Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes

article

Human Pancreatic Islet Isolation: Part II: Purification and Culture of Human Islets

article

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

article

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

article

Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection

article

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

article

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

article

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved