Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake

12.3K Views

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11:26 min

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September 21st, 2017

DOI :

10.3791/56093-v

September 21st, 2017

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Kathrine L. Jensen¹, Annika H. Runegaard¹, Pia Weikop², Ulrik Gether¹, Mattias Rickhag¹

¹Molecular Neuropharmacology and Genetics Laboratory, Lundbeck Foundation Center for Biomembranes in Nanomedicine, Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, ²Laboratory of Neuropsychiatry, Psychiatric Center Copenhagen and Department of Neuroscience and Pharmacology, University of Copenhagen

Summary

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Keywords Dopamine

Dopamine Transporter

HPLC

Synaptosomal Dopamine Uptake

Dopamine Homeostasis

In Vivo Manipulation

Brain Dissection

Tissue Homogenization

Synaptosome Preparation

Dopamine Uptake Assay

Chapters in this video

0:05

Title

1:00

Synaptosomal Preparation

3:22

Uptake Experiment

7:00

High-performance Liquid Chromatography Analysis

9:19

Results: Representative Results from a Synaptosomal DA Uptake Experiment of Four C57BI/6 Wild Type Mice from Drd1a-cre Mouse Strain

10:40

Conclusion

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