JoVE Logo
Faculty Resource Center

Sign In

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

DOI :

10.3791/56106-v

8:31 min

August 2nd, 2017

August 2nd, 2017

14,283 Views

1Centre for Stem Cells and Regenerative Medicine, King's College London, 2School of Molecular Biosciences, Washington State University

This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.

Tags

Keywords Horizontal Whole Mount

-- Views

Related Videos

article

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

article

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse

article

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

article

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

article

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

article

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

article

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

article

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

article

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

article

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved