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Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

DOI :

10.3791/56106-v

8:31 min

August 2nd, 2017

August 2nd, 2017

14,256 Views

1Centre for Stem Cells and Regenerative Medicine, King's College London, 2School of Molecular Biosciences, Washington State University

This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.

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Horizontal Whole Mount

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