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Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

DOI :

10.3791/57527-v

June 1st, 2018

June 1st, 2018

8,029 Views

1Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University

Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.

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Three dimensional Organotypic Cultures

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