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Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System

DOI :

10.3791/56144-v

August 17th, 2017

August 17th, 2017

14,154 Views

1School of Physics and Astronomy, University of Minnesota, 2Department of Diagnostic and Biological Sciences and Institute for Molecular Virology, University of Minnesota

A new generation of cell-free transcription-translation platforms has been engineered to construct biochemical systems in vitro through the execution of gene circuits. In this article, we describe how bacteriophages, such as MS2, ΦΧ174, and T7, are synthesized from their genome using an all E. coli cell-free TXTL system.

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Bacteriophages

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