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Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens

DOI :

10.3791/59495-v

8:52 min

March 14th, 2019

March 14th, 2019

16,658 Views

1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering

Cell-free expression systems are powerful and cost-efficient tools for the high-throughput synthesis and screening of important proteins. Here, we describe the preparation of cell-free protein expression system using Vibrio natriegens for the rapid protein production using plasmid DNA, linear DNA, and mRNA template.

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