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Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

DOI :

10.3791/56146-v

July 7th, 2017

July 7th, 2017

8,320 Views

1Department of Neuroscience, Karolinska Institutet, 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet

Robust derivation of human induced pluripotent stem (hiPS) cells was achieved by using non-integrating Sendai virus (SeV) vector mediated reprogramming of dermal fibroblasts. hiPS cell maintenance and clonal expansion was performed using xeno-free and chemically defined culture conditions with recombinant human laminin 521 (LN-521) matrix and Essential E8 (E8) Medium.

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Human Induced Pluripotent Stem Cells

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