JoVE Logo

Sign In

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

7.8K Views

10:48 min

November 26th, 2014

DOI :

10.3791/52158-v

November 26th, 2014


Explore More Videos

Keyword Extraction Human Induced Pluripotent Stem Cells

Chapters in this video

0:05

Title

1:49

Reprogramming Adult Human Dermal Fibroblasts with STEMCCA

4:55

Vector Integration Site Analysis and STEMCCA Excision

7:08

Conversion into GMP-grade Conditions

8:50

Results: Reprogramming hiPSCs Using the STEMCCA Lentiviral Approach

9:59

Conclusion

Related Videos

article

09:32

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

11.9K Views

article

09:45

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

10.6K Views

article

09:56

Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization

6.9K Views

article

10:48

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

8.5K Views

article

09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

9.8K Views

article

09:00

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

9.5K Views

article

13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

15.4K Views

article

10:24

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

21.4K Views

article

13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

19.9K Views

article

12:03

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

26.5K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved