Production of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex
12.8K Views
•
06:12 min
•
January 9th, 2018
DOI :
January 9th, 2018
•Rong Li*1, Jinxin Miao*1,2, Zhiqiang Fan1, SeokHwan Song3, Il-keun Kong3,4, Yaohe Wang2,5, Zhongde Wang1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2National Centre for International Research in Cell and Gene Therapy, Sino-British Research Centre, School of Basic Medical Sciences, Zhengzhou University, 3Department of Animal Science Division of Applied Life Science (BK21 Plus), Gyeongsang National University, 4Institute of Agriculture and Life Science, Gyeongsang National University, 5Centre for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London
Chapters in this video
0:05
Title
1:06
Zygote Isolation
3:31
PN Injection
4:52
Results: Evaluation of Gene Targeting in Golden Syrian Hamster by the Cas9/sgRNA System
5:36
Conclusion
Related Videos
08:22
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
14.7K Views
10:21
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
9.5K Views
10:19
Generation of Genetically Modified Mice through the Microinjection of Oocytes
20.4K Views
06:46
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
9.6K Views
08:30
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
2.5K Views
08:48
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
4.3K Views
10:57
CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
3.7K Views
07:17
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
2.8K Views
11:50
High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection
8.8K Views
09:40
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
95.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved