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Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

DOI :

10.3791/64161-v

8:48 min

June 24th, 2022

June 24th, 2022

2,911 Views

1Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba

The present protocol describes zygote microinjection of CRISPR-Cas9 and donor DNA to efficiently produce gene cassette knock-in and floxed mice.

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Zygote Microinjection

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