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Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

DOI :

10.3791/56346-v

4:24 min

November 22nd, 2017

November 22nd, 2017

9,532 Views

1Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 2Department of Health Sciences, Faculty of Applied Health Sciences, Brock University

This paper instructs users of in vivo micro-computed tomography (µCT) scanners how to anesthetize, correctly position and restrain the hind limb of a rat for minimal movement during high-resolution imaging of the tibia. The result is high quality images that can be processed to accurately quantify bone micro-architecture.

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Proper Positioning

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