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Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

DOI :

10.3791/56514-v

October 25th, 2017

October 25th, 2017

10,399 Views

1Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, 2Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne

We describe here a simple and quick method for the isolation of Salmonella typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin and streptavidin.

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Keywords Salmonella Typhimurium

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