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Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

DOI :

10.3791/56557-v

November 3rd, 2017

November 3rd, 2017

8,237 Views

1State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, 2University of Chinese Academy of Sciences

This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function.

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Aorta gonad mesonephros Explant Culture System

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