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An Ex vivo Culture System to Study Thyroid Development

DOI :

10.3791/51641-v

June 6th, 2014

June 6th, 2014

11,307 Views

1Pole of Cell Biology, Université catholique de Louvain & de Duve Institute

This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments.

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Keywords Thyroid Development

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