March 7th, 2018
•This protocol describes the combinatorial use of ChIP-seq, 4sU-seq, total RNA-seq, and ribosome profiling for cell lines and primary cells. It enables tracking changes in transcription-factor binding, de novo transcription, RNA processing, turnover and translation over time, and displaying the overall course of events in activated and/or rapidly changing cells.
Tags
Related Videos
In Vitro Transcription Assays and Their Application in Drug Discovery
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved