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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

DOI :

10.3791/51455-v

10:56 min

May 17th, 2014

May 17th, 2014

66,699 Views

1Lady Davis Institute and Department of Oncology, McGill University, 2Department of Oncology-Pathology, Karolinska Institutet, 3Goodman Cancer Centre and Department of Biochemistry, McGill University

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

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Polysome Fractionation

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