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Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

DOI :

10.3791/56799-v

January 2nd, 2018

January 2nd, 2018

8,979 Views

1Laboratorio de Oncología Molecular y TGF-β, IMIB-Arrixaca, 2Departamento de Enfermería, Facultad Enfermería, Universidad de Murcia

This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.

Tags

Microscopy

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