Primer-Free Aptamer Selection Using A Random DNA Library

24.6K Views

11:14 min

July 26th, 2010

DOI :

10.3791/2039-v

July 26th, 2010


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Primer Free Aptamer Selection

Chapters in this video

0:04

Title

0:12

Introduction

1:39

Preparation of PF Library-DNA Fragments

3:26

Preparation of S100B-bound Beads

4:55

Selection & Recovery of Aptamers

6:40

TOPO Cloning and Sequencing

7:17

Sandwich Binding Assays

9:11

Representative Results

10:35

Conclusion

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