Primer-Free Aptamer Selection Using A Random DNA Library

24.6K Views

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11:14 min

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July 26th, 2010

DOI :

10.3791/2039-v

July 26th, 2010

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Weihua Pan¹, Ping Xin¹, Susan Patrick¹, Stacey Dean², Christine Keating², Gary Clawson³^,⁴

¹Department of Pathology, Hershey Medical Center, Pennsylvania State University, ²Department of Chemistry, Pennsylvania State University, ³Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, ⁴Materials Research Institute, Pennsylvania State University

Summary

Chapters in this video

0:04

Title

0:12

Introduction

1:39

Preparation of PF Library-DNA Fragments

3:26

Preparation of S100B-bound Beads

4:55

Selection & Recovery of Aptamers

6:40

TOPO Cloning and Sequencing

7:17

Sandwich Binding Assays

9:11

Representative Results

10:35

Conclusion

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