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Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

DOI :

10.3791/56900-v

April 24th, 2018

April 24th, 2018

8,054 Views

1School of Life Science, University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

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