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A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila

DOI :

10.3791/62771-v

8:55 min

June 14th, 2021

June 14th, 2021

2,692 Views

1Department of Genetics and Biochemistry and Center for Human Genetics, Clemson University

The microplate feeder assay offers an economical, high throughput method for quantifying liquid food consumption in Drosophila. A 3D-printed device connects a 96-well microplate in which flies are housed to a 1536-well microplate from which flies consume a feeding solution with a tracer dye. The solution volume decline is measured spectrophotometrically.

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High throughput

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