The overall goal of this procedure is to quantitatively measure the food-intake of adult Drosophila, which provides a means to evaluate the effects of genetic manipulations such as thermogenetic and optogenetic activations of neurons. These measur
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Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.