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Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

DOI :

10.3791/56949-v

March 2nd, 2018

March 2nd, 2018

9,979 Views

1Department of Pathology, Feinberg School of Medicine, Northwestern University

New protocols are described here to isolate and characterize microparticles derived from human and mouse neutrophils. These protocols utilize ultracentrifugation, flow cytometry, and immunoblotting techniques to analyze microparticle content, and they can be used to study the role of microparticles derived from various cell types in cellular function.

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Keywords Neutrophil derived Microparticles

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