Murine Bone Marrow Polymorphonuclear (PMN) Cell Separation
2:37
Microparticle (MP) Isolation from Bone Marrow-derived Activated Murine PMNs
3:45
In Vivo PMN-derived Microparticle (MP) Wound Healing Assay
4:38
Results: Representative Effects of Isolated PMN-MPs in Epithelial Wound Healing
5:36
Conclusion
Transkript
The overall goal of this protocol is to isolate and characterize neutrophil-derived microparticles for functional studies. This method can help answer key questions about the role of exo-cell vesicles, or microparticles, in cell-cell communication
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New protocols are described here to isolate and characterize microparticles derived from human and mouse neutrophils. These protocols utilize ultracentrifugation, flow cytometry, and immunoblotting techniques to analyze microparticle content, and they can be used to study the role of microparticles derived from various cell types in cellular function.