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Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

DOI :

10.3791/56955-v

May 29th, 2018

May 29th, 2018

9,927 Views

1Knock Out Mouse Core, NIEHS/NIH

Here we present a protocol to increase chimera production without the use of new equipment. A simple orientation change of the embryo for injection can increase the number of embryos produced, and potentially reduce the timeline to germline transmission.

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Trans inner Cell Mass Injection

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