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Derivation of Human Embryonic Stem Cells by Immunosurgery

33.8K Views

11:56 min

December 13th, 2007

DOI :

10.3791/574-v

December 13th, 2007


Transcript

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Human Embryonic Stem Cells

Chapters in this video

0:07

Introduction

1:17

Preparation of plates for immunosurgery

2:34

Preparing capillary pipettes

3:32

Immunosurgery to isolate Inner Cell Mass (ICM)

6:30

Development of human ES cell outgrowth

6:42

Passaging outgrowth

7:49

Discussion: Technical aspects of human ES cell derivation

9:12

Heterogeneity of human ES cell lines: implications for development

10:53

Advances in human ES cell derivation

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