Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

13.8K Views

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09:01 min

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March 26th, 2018

DOI :

10.3791/56976-v

March 26th, 2018

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Thomas Boyer¹^,², Fanny Gonzales²^,³, Adriana Plesa⁴, Pauline Peyrouze², Adeline Barthelemy², Soizic Guihard², Bruno Quesnel²^,⁵, Christophe Roumier¹^,², Claude Preudhomme¹^,², Meyling Cheok²

¹Laboratoire d’hématologie, CHU Lille, ²Univ. Lille, Inserm, CHU Lille, UMR-S 1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, ³Service d’hématologie pédiatrique, hôpital Jeanne de Flandre, CHU Lille, ⁴Laboratoire d’hématologie CH Lyon Sud, ⁵Service des Maladies du Sang, hôpital Claude Huriez, CHU Lille

Summary

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Keywords Flow Cytometry

Leukemia Stem Cells

Acute Myeloid Leukemia

Patient derived Xenografts

Submandibular Blood Collection

Chapters in this video

0:04

Title

0:58

Sample Preparation and Lysis of Red Blood Cells

2:26

Patient Derived Xenografts and Staining

4:50

Flow Cytometry and Gating Strategy

6:14

Results: Parametric Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia

7:57

Conclusion

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