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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

DOI :

10.3791/64918-v

10:20 min

March 24th, 2023

March 24th, 2023

698 Views

1Quantitative Immuno-hematology, CNRS UMR168, Institut Curie, 2Hamilton Institute, Maynooth University, 3Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, 4Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.

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Flow Cytometry

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