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Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

DOI :

10.3791/57065-v

February 16th, 2018

February 16th, 2018

18,626 Views

1The Ritchie Centre, Hudson Institute of Medical Research, 2Department of Obstetrics & Gynaecology, Monash University

A protocol for the isolation of primary microglia from murine brains is presented. This technique aids in furthering the current understanding of neurological conditions. Density gradient centrifugation and magnetic separation are combined to produce sufficient yield of a highly pure sample. Furthermore, we outline the steps for characterization of microglia.

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