April 13th, 2017
•Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.
Related Videos
The Use of Magnetic Resonance Spectroscopy as a Tool for the Measurement of Bi-hemispheric Transcranial Electric Stimulation Effects on Primary Motor Cortex Metabolism
Effects of Transcranial Alternating Current Stimulation on the Primary Motor Cortex by Online Combined Approach with Transcranial Magnetic Stimulation
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
Rapid and Specific Immunomagnetic Isolation of Mouse Primary Oligodendrocytes
Rapid Isolation of Dorsal Root Ganglion Macrophages
Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved