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Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

DOI :

10.3791/57122-v

March 9th, 2018

March 9th, 2018

15,286 Views

1Department of Neurobiology, Stanford University

Efforts to understand microglial function in detail have been hindered by the lack of microglial culture models that recapitulate the properties of mature in vivo microglia. This protocol describes an isolation and culture approach designed to maintain robust survival of highly ramified mature rat microglia under defined-medium conditions.

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Keywords Microglia

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