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Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

15.8K Views

12:00 min

March 9th, 2018

DOI :

10.3791/57122-v

March 9th, 2018


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Microglia

Chapters in this video

0:04

Title

0:41

Tissue Collection and Mechanical Dissociation

2:52

Myelin Removal

4:07

Immunopanning

7:56

Spot Coating Tissue Culture/Coverslips

9:22

Results: Defined-medium Microglia Cultures Show Ramified Morphology with Dynamic Processes

11:22

Conclusion

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