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CRISPR Guide RNA Cloning for Mammalian Systems

DOI :

10.3791/57998-v

6:48 min

October 2nd, 2018

60,635 Views

1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute

Here, a simple, efficient, and cost-effective method of sgRNA cloning is outlined.

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