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CRISPR Guide RNA Cloning for Mammalian Systems

DOI :

10.3791/57998-v

6:48 min

October 2nd, 2018

October 2nd, 2018

62,187 Views

1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute

Here, a simple, efficient, and cost-effective method of sgRNA cloning is outlined.

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CRISPR

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