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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

DOI :

10.3791/57479-v

9:02 min

April 20th, 2018

April 20th, 2018

18,990 Views

1Cluster of excellence CellNetworks, Heidelberg University, 2Heidelberg University Biochemistry Center (BZH)

We provide a step-by-step protocol for split-BioID, a protein fragments-complementation assay based on the proximity-labeling technique BioID. Activated on the interaction of two given proteins, it allows the proteomics analysis of context-dependent protein complexes in their native cellular environment. The method is simple, cost-effective and only requires standard laboratory equipment.

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Keywords Split BioID

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