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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

DOI :

10.3791/58952-v

February 12th, 2019

February 12th, 2019

10,732 Views

1Cellular Protein Chemistry, Utrecht University, 2Program in Cellular and Molecular Medicine, Boston Children's Hospital

Here we describe a protocol for a general pulse-chase method that allows the kinetic analysis of folding, transport, and degradation of proteins to be followed in live cells.

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Keywords Protein Folding

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