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Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids Video (Video) | JoVE

DOI :

10.3791/57551-v

11:56 min

May 4th, 2018

May 4th, 2018

11,953 Views

1Department of Biocatalysis, Institute of Chemistry, Technische Universität Berlin, 2Department of Bioenergetics, Institute of Chemistry, Technische Universität Berlin, 3Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Department of Molecular Genetics, University of Groningen

The protocol presents the Escherichia coli-based selective pressure incorporation of non-canonical amino acids (ncAAs) into the lactococcal antimicrobial peptide nisin. Its properties can be changed during recombinant expression via substitution with desired ncAAs in defined growth media. Resulting changes in bioactivity are mapped by growth inhibition assays and fluorescence microscopy.

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Antimicrobial Peptides

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