Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

11.8K Views

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11:51 min

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April 27th, 2018

DOI :

10.3791/57017-v

April 27th, 2018

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Tobias Baumann¹, Franz-Josef Schmitt², Almut Pelzer¹, Vivian Jeanette Spiering³, Georg Johannes Freiherr von Sass¹, Thomas Friedrich², Nediljko Budisa¹

¹Institute of Chemistry L 1, Department of Biocatalysis, Technical University of Berlin, ²Institute of Chemistry PC 14, Department of Bioenergetics, Technical University of Berlin, ³Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences, Technical University of Berlin

Summary

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Keywords Selective Pressure Incorporation

Non canonical Amino Acids

Recombinant Protein Production

Chapters in this video

0:05

Title

1:46

Recombinant Protein Expression

4:05

Target Protein Purification via Immobilized Metal Ion Affinity Chromatography

5:20

Intact Protein Mass Analysis by High-performance Liquid Chromatography Coupled to Electrospray Ionization Time-of-flight Mass Spectrometry

6:38

Fluorescence Lifetime Measurements and Decay-associated Spectra of GdFP

7:59

Results: Gold Fluorescent Protein Analysis by Mass Spectrometry and Fluorescence

10:13

Conclusion

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