Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11.9K Views
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11:51 min
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April 27th, 2018
DOI :
April 27th, 2018
•Tobias Baumann1, Franz-Josef Schmitt2, Almut Pelzer1, Vivian Jeanette Spiering3, Georg Johannes Freiherr von Sass1, Thomas Friedrich2, Nediljko Budisa1
1Institute of Chemistry L 1, Department of Biocatalysis, Technical University of Berlin, 2Institute of Chemistry PC 14, Department of Bioenergetics, Technical University of Berlin, 3Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences, Technical University of Berlin
Chapters in this video
0:05
Title
1:46
Recombinant Protein Expression
4:05
Target Protein Purification via Immobilized Metal Ion Affinity Chromatography
5:20
Intact Protein Mass Analysis by High-performance Liquid Chromatography Coupled to Electrospray Ionization Time-of-flight Mass Spectrometry
6:38
Fluorescence Lifetime Measurements and Decay-associated Spectra of GdFP
7:59
Results: Gold Fluorescent Protein Analysis by Mass Spectrometry and Fluorescence
10:13
Conclusion
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