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Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

DOI :

10.3791/57615-v

July 15th, 2018

July 15th, 2018

8,219 Views

1Institute for Advanced Biosciences, Keio University, 2Systems Biology Program, Graduate School of Media and Governance, Keio University, 3Faculty of Environment and Information Studies, Keio University

Contamination during the genomic sequencing of microscopic organisms remains a large problem. Here, we show a method to sequence the genome of a tardigrade from a single specimen with as little as 50 pg of genomic DNA without whole genome amplification to minimize the risk of contamination.

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