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An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

1.9K Views

11:36 min

April 21st, 2023

DOI :

10.3791/65316-v

April 21st, 2023


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LiCHi C

Chapters in this video

0:00

Introduction

1:44

Lysis and Digestion of Scarce Cells

2:54

Ligation and Decrosslinking of Scarce Cell DNA

3:55

Sonication and Biotin Pull‐Down of Scarce Cells DNA

5:36

"dATP‐Tailing, Adapter Ligation,and PCR Amplification of Scarce Cell DNA"

9:04

"Library Capture, Biotin Pull‐Down, and PCR Amplification Using Scarce Cell DNA Library"

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