Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

7.9K Views

•

09:38 min

•

June 26th, 2019

DOI :

10.3791/59329-v

June 26th, 2019

•

Jerlisa Ann C. Arizala¹^,², Pritsana Chomchan¹, Haitang Li¹, Roger Moore³, Helen Ge³, Dominique L. Ouellet¹, John J. Rossi¹^,²

¹Molecular and Cellular Biology Department, Beckman Research Institute at the City of Hope, ²Irell & Manella Graduate School of Biological Sciences, ³Department of Molecular Immunology, Beckman Research Institute and the City of Hope

Summary

Explore More Videos

HIV 1 Replication

Nucleolar Factors

Rev Immunoprecipitation

Mass Spectrometry

Viral Host Factors

HeLa Cell Line

Transfection Protocol

Lysis Buffer

Protease Inhibitor Cocktail

Molecular Immunology

Beckman Research Institute

Cell Culture Techniques

HLfB Culture Plates

Protein Characterization

Chapters in this video

0:04

Title

0:47

Rev-nucleolar Localization Signal (NoLS)-3'Flag Mutation Expression During HIV-1 Replication

2:03

Viral Protein Lysate Collection

3:12

Rev-NoLS-3'Flag Coimmunoprecipitation

5:20

In-gel Reduction, Alkylation, and Digestion of Coomassie-stained Gel Bands

7:14

Results: Representative Nucleolar Factor Identification During HIV-1 Replication

9:05

Conclusion

Related Videos

09:53

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein

12.9K Views

11:10

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

12.2K Views

10:49

In vitro Uncoating of HIV-1 Cores

15.6K Views

09:27

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

11.1K Views

14:23

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

15.5K Views

13:29

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

24.1K Views

07:10

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

14.3K Views

08:33

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

8.8K Views

06:46

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

17.2K Views

08:21

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

8.8K Views