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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

9.2K Views

10:46 min

June 2nd, 2018

DOI :

10.3791/57883-v

June 2nd, 2018


Explore More Videos

CRISPR

Chapters in this video

0:05

Title

0:37

Guide RNA Design

3:37

Guide RNA Cloning

6:27

Transfection of Enhancer-I, Cell Harvest, and RNA Extraction

8:21

Results: Guide RNA Design/Cloning and Evaluation of Gene Expression in Response to Enhancer-i

9:23

Conclusion

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