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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

DOI :

10.3791/57883-v

10:46 min

June 2nd, 2018

June 2nd, 2018

8,868 Views

1Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah

This protocol describes the steps needed to design and perform multiplexed targeting of enhancers with the deactivating fusion protein SID4X-dCas9-KRAB, also known as enhancer interference (Enhancer-i). This protocol enables the identification of enhancers that regulate gene expression and facilitates the dissection of relationships between enhancers regulating a common target gene.

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Enhancer Function

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