July 30th, 2018
•This protocol demonstrates how to track a protein nuclear translocation under heat stress by using a green fluorescence protein (GFP) fusion protein as a marker and 4',6-diamidino-2-phenylindole (DAPI) staining. The DAPI staining protocol is fast and preserves the GFP and protein subcellular localization signals.
Tags
Related Videos
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
Basic Caenorhabditis elegans Methods: Synchronization and Observation
Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved