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RNA Fluorescence in situ Hybridization (FISH) to Visualize Microbial Colonization and Infection in Caenorhabditis elegans Intestines

DOI :

10.3791/63980-v

8:58 min

July 27th, 2022

July 27th, 2022

2,479 Views

1Department of Biology, San Diego State University, 2School of Biological Sciences, University of California, San Diego

Intestinal microbes, including extracellular bacteria and intracellular pathogens like the Orsay virus and microsporidia (fungi), are often associated with wild Caenorhabditis nematodes. This article presents a protocol for detecting and quantifying microbes that colonize and/or infect C. elegans nematodes, and for measuring pathogen load after controlled infections in the lab.

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RNA Fluorescence In Situ Hybridization

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