Name: a versatile model of hard tick infestation on laboratory rabbits
Uploaded: 2018-10-06T15:00:00.000+00:00
Duration: 5 min 38 s
Description: Watch this Scientific Journal Video about A Versatile Model of Hard Tick Infestation on Laboratory Rabbits at JoVE.com

We acknowledge the technical assistance of Evelyne Le Naour, French National Institute of Agricultural Research (INRA), Alain Bernier (INRA) and Oc&#233;ane Le Bidel (ANSES). Consuelo Almaz&#225;n was supported by a fellowship from the Laboratory of Excellence, Integrative Biology of Emerging Infectious Diseases (LabEx IBEID), Pasteur Institute. The rabbits and sheep were purchased by ANSES. Part of this work was funded by National Institutes of Health grant RO1AI090062 to Y. Park. Dr. Jeffrey L. Blair is acknowledged for reviewing the manuscript.

NOTE: In this study, rabbits were maintained in standard cages with food and water offered ad libitum at the French Agency for Food, Environmental and Occupational Health &#38; Safety (ANSES) accredited animal facilities in Maisons-Alfort, France. Animals were monitored twice daily by two experienced technicians for any abnormal skin reactions, health problems or complications. The experimental room was secured by framing the interior of the doorway with double-sided tape to avoid accidental escape of ticks. The method works best if two people work as a team, but it is possible to complete single handedly by one experienced person. Although most rabbits are docile and calm, signs of stress may occur during the manipulation. To ensure that a rabbit does not injure itself by struggling, manual restraint may be accomplished by gently holding of the scruff of the neck in one hand while the other hand supports it hind quarters. The six month old, Rambouillet female sheep was kept at the Centre for Biomedical Research (CRBM) facilities at the National Veterinary School of Alfort (ENVA), where water and food were supplied ad libitum, and it was checked twice daily.
NOTE: Our laboratory has received permission to use rabbits and sheep for tick feeding by the Ethics Committee for Animal Experiments ComEth Anses/ENVA/UPEC, Permit Numbers 01741.01 and 11/10/16-5B, respectively. Since we used only pathogen-free ticks in our experiments, all the rabbits used in this study were offered for adoption via the White Rabbit Association, Paris, France.
1. Preparation of the Capsules
<ol>
	<li>Cut the desired size of the capsule from the EVA foam sheet (Figure 1A). Round the outer corners (Figure 1B) of the capsule to minimize accidental detachment when gluing it to the rabbit skin. 
	NOTE: The frame thickness of the capsule should be around 8 mm. Use a 5 mm thick foam sheet for the larvae, nymphs, and small tick adult species such as Ixodes. A 1 cm thickness foam sheet is suitable for large size adult ticks such as Amblyomma sp., Hyalomma sp., etc. The size of the capsule varies based on the experimental requirements. For example, for 20 Ixodes adult couples, 200 nymphs, or 1,000 larvae, we use an inner capsule size of 5 x 5 cm2, 6 x 7 cm2 or 7 x 9 cm2, respectively.</li>
	<li>Cut the 8 mm wide strips of self-adhesive hook tape (see Table of Materials) and stick them to the prepared EVA foam frame (Figure 1C).</li>
	<li>Cut the same size strips from self-adhesive loop tape (see Table of Materials) and bind them to the hook sides attached to the EVA-foam frame (Figure 1D).</li>
	<li>Cut the appropriate size of the fine mosquito mesh (mesh size less than 50 &#181;m) to the size of the EVA foam frame and stick it to the self-adhesive loop (Figure&#160;1E and 1F). Cut the overhangs if necessary. 
	NOTE: This type of capsule can be used to feed nymphs and adults of hard tick species, while a different sealing system of the capsules is needed for larval feeding (Supplemental Figure 1) to prevent accidental escape of the larvae via the fastened hook-and-loop side.</li>
</ol>
2. Preparation of the Rabbit before Tick Infestation
<ol>
	<li>Shave the area of the rabbit back and sides to be used with clippers (Figure 2A).</li>
	<li>Apply non-irritating latex glue to the entire surface of the prepared capsule and wait for 1 min (Figure 2B).</li>
	<li>Glue the capsule by pressing to the skin (especially at the corners) with the fingers for about 3 minutes (Figure&#160;2C and 2D). 
	NOTE: When gluing more than one capsule, make sure to keep at least 5 mm space between them (Figure&#160;2E and 2F). We usually avoid the region of the spine, but it may be used if needed.</li>
	<li>Slightly lift the capsules to visually check their attachment to the skin. If non-attached regions are found, apply the glue using a spatula and press for another 3 minutes.</li>
	<li>Apply protective tape to the rear paws of the rabbit to prevent jacket damage (Figure 2G). 
	NOTE: This step is optional and is mainly to prevent jacket damage, not damage to the tick capsule.</li>
	<li>Put on the rabbit jacket by placing the front legs through the openings and tightening the neck,&#160;making sure rabbit breathing remains comfortable. Do not place the rear legs through the elastic enclosures at this step and leave the zipper open (Figure 2H).</li>
</ol>
3. Tick Infestation
<ol>
	<li>Place the ticks into a plastic syringe (1 or 5 mL depending of the number of the individuals) with the needle-end cut and plugged with cotton (Figure 1G). If a small amount of ticks are to be infested, use forceps. 
	NOTE: For tick colony maintenance allow the engorged tick female(s) to lay eggs with subsequent hatching inside the syringe (5 or 10 mL) covered by the mosquito mesh wrapped by a rubber band12 to avoid laborious manipulation of the larvae at the time they are applied to the host (Figure 1H). Also, fully engorged larvae may be allowed to molt in the syringe (Supplemental Figure 1I; 5 or 10 mL) for direct infestation of the rabbit with nymphs.</li>
	<li>Place the syringe deep into the capsule via the open corner and inoculate the ticks by pushing the syringe plunger. Slowly twist the plunger toward the rabbit skin to remove the remaining ticks attached to the plunger and simultaneously pull it out from the capsule (Figure 2I). 
	NOTE: If some of the individuals crawl out of the capsule, return them using forceps.</li>
	<li>Close the capsule by refastening the hook-and-loop tape.</li>
	<li>Place the rear legs of the rabbit into the rear elastic enclosures of the jacket and zip closed. 
	NOTE: Make that an index finger can fit between the neck of the jacket and the rabbit to ensure comfort and also to avoid chewing on the jacket.</li>
	<li>Return the rabbit to the cage (Figure 2J). 
	NOTE: The time from the infestation to collection of replete ticks vary among different tick species and the developmental stages. For example, for Ixodes scapularis and Ixodes ricinus, the durations of feeding for adults, nymphs, and larvae are 6&#8211;9, 3&#8211;4, and 2&#8211;3 days, respectively. A list of references for 29 different hard tick life cycles under laboratory conditions can be found in Levin and Shumacher (2016)9.</li>
</ol>
4. Collection and Monitoring of Ticks
<ol>
	<li>Take the rabbit from the cage to the bench and unzip the jacket.</li>
	<li>Gently restrain the rabbit with your hands. Open the capsule by unfastening the hook-and-loop tape (Figure&#160;2K and 2L) and collect the ticks by brushing the engorged larvae (Supplemental Figure 1) or nymphs to a plastic dish or using forceps for adults (Figure 2L). If partially fed (not replete) ticks are required, use a tick twister or forceps to detach them. 
	NOTE: If maintaining the tick colonies please see the note in step 3.1. Maintain the engorged ticks in appropriate humid and temperature conditions according to the particular tick species.</li>
	<li>If needed, refasten the hook-and-loop tape to close the capsule.</li>
</ol>
5. Recovery of the Rabbit
<ol>
	<li>Remove the mosquito mesh completely from the capsule and leave the jacket on the rabbit (Figure 2M).</li>
	<li>Wait 3-4 weeks and try to remove the capsule by gently trimming one of the corners (Figure 2N). If the capsule is still firmly attached, repeat this step one week later.</li>
	<li>Remove the jacket and let the rabbit recover in the cage. 
	NOTE: Once the capsule is off, check the skin of the rabbit for abnormal reactions. Although normally no treatment is required, an emollient lotion can be used in case of irritation.</li>
	<li>If the protocol and experiments allow, the recovered rabbit (Figure 2O) can be reused or offered for adoption. 
	NOTE: Rabbits have been shown to acquire tick resistance once exposed to repeated tick infestations13; therefore, reinfestations are not recommended unless the experiment requires.</li>
</ol>

<ol>
	<li>Sonenshine, D. E., Roe, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ticks,+People+and+Animals.">Ticks, People and Animals.</a> Biology of Ticks, Vol I. , (2014).</li><li>Kr&#246;ber, T., Guerin, P. 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M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick+rearing+methods+with+special+reference+to+the+Rocky+Mountain+Wood+Tick,+Dermacentor+andersoni+Stiles.">Tick rearing methods with special reference to the Rocky Mountain Wood Tick, Dermacentor andersoni Stiles.</a> Culture methods for invertebrate animals. , (1937).</li><li>Faccini, J. L. H., Chacon, S. C., Labruna, M. 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The authors have nothing to disclose.

The most important step in this entire protocol is to glue the capsule firmly to the shaved skin. For this reason, constant pressure for at least 3 minutes is critical, especially at the corners. When inoculating the ticks into the capsule, it is important to apply them deep into the opposite corner from the open one to avoid tick escape during the sealing. When planning the experiments, make sure that all the capsules are covered by the jacket to avoid damage by chewing or scratching. Make sure that the neck region of t...

The hard ticks (Ixodidae) are well known as slow feeding arthropods and can be attached on a host for several days, or weeks, depending on the species and developmental stage1. These obligatory hematophagous arthropods are vectors of a wide variety of infectious agents, such as bacteria, protozoa, and viruses, and thus present a significant risk to humans and animal health1. When studying tick biology or evaluating new control methods, the establishment of an effective tick feeding system is crucial in order to effectively design the experiments and accomplish the goal(s) of the study. Recently, several artificial tick feeding systems (avoiding the use of live animals) have been developed2,3,4 and they should be used whenever possible. However, these systems have not been able to completely replace tick feeding on live animals, and they are not suitable substitutes for many physiologic conditions required for scientific studies. Therefore, in some cases, the use of experimental animal hosts is crucial to guarantee the relevance of experimental results.
Laboratory New Zealand rabbits have shown to be the most suitable and accessible hosts for several ixodid tick species5,6,7,8,9. Two common strategies of tick feeding on rabbits have been frequently used: a) feeding on rabbit ears covered with cotton cloth or socks6,7, and b) feeding in cotton bags9, nylon bottles10 or neoprene chambers11&#160;glued to the rabbit&#39;s back. The feeding on rabbit&#39;s ears is not an elegant system, because ticks (especially early stages, larvae or nymphs) may crawl and attach deep in the ear canal, which is uncomfortable for the animal and makes the monitoring of tick feeding and/or the recovery of engorged ticks difficult. This system is also limited to only two tick groups on ears fully covered by socks protected by Elizabethan collars,representing a significant discomfort for the animal. Other systems9,10,11 are definitely more advanced and well suited for hard tick colony maintenance. However, they are limited in the number of experimental groups placed on the rabbit as well as in the modifiable sizes/shapes of the feeding chambers. In addition, these protocols often require hobbling the rear rabbit legs to avoid scratching and the use of Elizabethan collars to prevent grooming.
Herein we propose a simple, non-laborious and very effective method to feed multiple groups of hard ticks in closed chambers glued to the rabbit back covered by a jacket, eliminating the need for Elizabethan collars or hobbling during the experiment. Specifically, our system uses elastic capsules made from an ethylene-vinyl acetate (EVA) foam sheet covered by mosquito mesh and glued to the shaved rabbit back with fast solidifying (3 min) non-irritating latex glue. This technique allows the attachment of multiple capsules of desired size or shape, and few weeks after the experiment the rabbits are fully recovered. The system is suitable mainly for the nymphal and adult hard tick stages, but with a little modification it can be used for larvae feeding as well. The EVA foam-based methods for hard tick feeding may be adapted to other types of vertebrate hosts, for example sheep (which is shown as one of the alternatives in this paper).

<table><tbody><tr><td>New Zealand Rabbits (2.5-3.5 kg)</td><td>Charles River&amp;nbsp;</td><td>Strain Code 571</td><td></td></tr><tr><td>Rambouillet sheep</td><td>Local provider-tick free farm</td><td>Female 6 months old</td><td></td></tr><tr><td>EVA foam 5 mm thick&amp;nbsp;</td><td>Cosplay Shop</td><td>EVA-PE451kg (950mm x 450mm)</td><td>10 mm PE45 kg foam from the Cosplay Shop may be used for the large adult tick species</td></tr><tr><td>Foam Sheet 9&quot; X 12&quot; 6 mm-White</td><td>Amazon</td><td>FOAMSHT6-20</td><td>6 mm-EVA foam ca be ordered via Amazon as an alternative to the foam from Cosplay Shop</td></tr><tr><td>Full length rabbit jackets&amp;nbsp;</td><td>Harvard Apparatus, Inc.&amp;nbsp;</td><td>620077- medium, 6270078 - large&amp;nbsp;</td><td></td></tr><tr><td>Non-toxic latex glue&amp;nbsp;</td><td>Tear mender&amp;nbsp;</td><td>Fabric &amp;amp; Leather Adhesive</td><td></td></tr><tr><td>Tubular cotton orthopedic stockinette</td><td>BSN Medical</td><td>9076 (12-15 cm wide)</td><td></td></tr><tr><td>Mosquito mesh&amp;nbsp;</td><td>Loisirs Creatifs</td><td>Very fine filter nylon mesh fabric</td><td>Any mosquito mesh, or curtain material with the mesh size less than 50 &amp;mu;m is suitable.</td></tr><tr><td>Leukoplast&amp;nbsp;</td><td>BSN medical S.A.S</td><td>LF 72361-02</td><td></td></tr><tr><td>Adhesive hook-and-loop tape</td><td>AIEX store</td><td>AIEX 39.37 Feet/12m Hook and Loop Self Adhesive Tape Roll, 20 mm width, white colour</td><td>Fullfiled by Amazon</td></tr><tr><td>Fast drying glue &amp;nbsp;</td><td>Fixtout&amp;nbsp;</td><td>Superglue</td><td></td></tr></tbody></table>

a versatile model of hard tick infestation on laboratory rabbits

The use of live animals in tick research is crucial for a variety of experimental purposes including the maintenance of hard tick colonies in the laboratory. In ticks, all developmental stages (except egg) are hematophagous, and acquiring a blood-meal when attached to their vertebrate hosts is essential for the successful completion of their life cycle. Here we demonstrate a simple method that uses easily openable capsules for feeding of hard ticks on rabbits. The advantages of the proposed method include its simplicity, short duration and most importantly versatile adjustment to the needs of specific experimental requirements. The method makes possible the use of multiple chambers (of various sizes) on the same animal, which permits feeding of multiple stages or different experimental groups while reducing the overall animal requirement. The non-irritating and easily accessible materials used minimizes discomfort to the animals, which can be easily recovered from an experiment and offered for adoption or reused if the ethical protocol allows it.

Here we propose for the first time a detailed step-by step method of hard tick feeding in EVA foam capsules applied to a shaved rabbit&#39;s back, covered by a jacket (Figure&#160;1 and&#160;Figure 2). This protocol is suitable for various types of experiments when different tick groups on the same host are required and can be also used for mass rearing of hard ticks. The tick feeding success in the laboratory mo...

Watch this Scientific Journal Video about A Versatile Model of Hard Tick Infestation on Laboratory Rabbits at JoVE.com

A Versatile Model of Hard Tick Infestation on Laboratory Rabbits

We have developed a simple and versatile system to feed hard ticks on laboratory rabbits. Our non-laborious protocol uses easily accessible materials and can be adjusted depending on the requirements of the various experimental settings. The method allows comfortable monitoring and/or sampling of ticks during the entire feeding period.

The use of live animals in tick research is crucial for a variety of experimental purposes including the maintenance of hard tick colonies in the ...

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10.9K Views.  Université Paris-Est. We have developed a simple and versatile system to feed hard ticks on laboratory rabbits. Our non-laborious protocol uses easily accessible materials and can be adjusted depending on the requirements of the various experimental settings. The method allows comfortable monitoring and/or sampling of ticks during the entire feeding period.

Video: A Versatile Model of Hard Tick Infestation on Laboratory Rabbits

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Internal and external parasites remain a significant concern in laboratory rodent facilities, and many research facilities harbor some parasitized animals. Before embarking on an examination of animals for parasites, two things should be considered. One: what use will be made of the information collected, and two: which test is the most appropriate. Knowing that animals are parasitized may be something that the facility accepts, but there is often a need to treat animals and then to determine the efficacy of treatment. Parasites may be detected in animals through various techniques, including samples taken from live or euthanized animals. Historically, the tests with the greatest diagnostic sensitivity required euthanasia of the animal, although PCR has allowed high-sensitivity testing for several types of parasite. This article demonstrates procedures for the detection of endo- and ectoparasites in mice and rats. The same procedures are applicable to other rodents, although the species of parasites found will differ.

This article describes various procedures for screening rats and mice to detect endo- or ectoparasitism. Several diagnostic assays will be demonstrated, both those suitable for use on live animals and those used after euthanasia of the animal. Photographs to aid in identification of rat and mouse parasites will be included.

Internal and external parasites remain a significant concern in laboratory rodent facilities, and many research facilities harbor some parasitized ...

Immunology and Infection

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Traditionally, hamsters are experimentally inoculated in the snout or the footpad. However in these sites an ulcer not always occurs, measurement of lesion size is a hard procedure and animals show difficulty to eat, breathe and move because of the lesion. In order to optimize the hamster model for cutaneous leishmaniasis, young adult male and female golden hamsters (Mesocricetus auratus) were injected intradermally at the dorsal skin with 1 to 1.5 x l07 promastigotes of Leishmania species and progression of subsequent lesions were evaluated for up to 16 weeks post infection. The golden hamster was selected because it is considered the adequate bio-model to evaluate drugs against Leishmania as they are susceptible to infection by different species. Cutaneous infection of hamsters results in chronic but controlled lesions, and a clinical evolution with signs similar to those observed in humans. Therefore, the establishment of the extent of infection by measuring the size of the lesion according to the area of indurations and ulcers is feasible. This approach has proven its versatility and easy management during inoculation, follow up and characterization of typical lesions (ulcers), application of treatments through different ways and obtaining of clinical samples after different treatments. By using this method the quality of animal life regarding locomotion, search for food and water, play and social activities is also preserved.

Optimization of the experimental hamster model for cutaneous leishmaniasis by intradermal injection of Leishmania promastigotes at the dorsal skin. This approach is useful during inoculation, follow-up, characterization of lesions, application of treatments and obtaining of clinical samples. Locomotion, search for food and water, play and social activities are preserved.

Traditionally, hamsters are experimentally inoculated in the snout or the footpad. However in these sites an ulcer not always occurs, measurement of ...

Tickling, a Technique for Inducing Positive Affect When Handling Rats

Handling small animals such as rats can lead to several adverse effects. These include the fear of humans, resistance to handling, increased injury risk for both the animals and the hands of their handlers, decreased animal welfare, and less valid research data. To minimize negative effects on experimental results and human-animal relationships, research animals are often habituated to being handled. However, the methods of habituation are highly variable and often of limited effectiveness. More potently, it is possible for humans to mimic aspects of the animals&#39; playful rough-and-tumble behavior during handling. When applied to laboratory rats in a systematic manner, this playful handling, referred to as tickling, consistently gives rise to positive behavioral responses. This article provides a detailed description of a standardized rat tickling technique. This method can contribute to future investigations into positive affective states in animals, make it easier to handle rats for common husbandry activities such as cage changing or medical/research procedures such as injection, and be implemented as a source of social enrichment. It is concluded that this method can be used to efficiently and practicably reduce rats&#39; fearfulness of humans and improve their welfare, as well as reliably model positive affective states.

This article demonstrates the standardized application of playful handling, a tickling technique designed to mimic rat rough-and-tumble play. This technique is effective at reducing fearful reactions to humans and generating positive affect when rats are handled for common husbandry activities and medical and research procedures such as injection.

Handling small animals such as rats can lead to several adverse effects. These include the fear of humans, resistance to handling, increased injury risk ...

Behavior

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the attachment and blood feeding of Ixodes species ticks on mammalian hosts. In nature, this zoonotic bacterial pathogen may use a variety of reservoir hosts, but the white-footed mouse (Peromyscus leucopus) is the primary reservoir for larval and nymphal ticks in North America. Humans are incidental hosts most frequently infected with B. burgdorferi by the bite of ticks in the nymphal stage. B. burgdorferi adapts to its hosts throughout the enzootic cycle, so the ability to explore the functions of these spirochetes and their effects on mammalian hosts requires the use of tick feeding. In addition, the technique of xenodiagnosis (using the natural vector for detection and recovery of an infectious agent) has been useful in studies of cryptic infection. In order to obtain nymphal ticks that harbor B. burgdorferi, ticks are fed live spirochetes in culture through capillary tubes. Two animal models, mice and nonhuman primates, are most commonly used for Lyme disease studies involving tick feeding. We demonstrate the methods by which these ticks can be fed upon, and recovered from animals for either infection or xenodiagnosis.

Lyme disease is the most commonly-reported vector-borne disease in North America. The causative agent, Borrelia burgdorferi is a spirochete bacterium transmitted by Ixodid ticks. Transmission and detection of infection in animal models is optimized by the use of tick feeding, which we describe here.

Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

Positive Reinforcement-Based Training for Routine Rabbit Husbandry Procedures

Training Laboratory Rabbits to Refine Routine Husbandry Procedures

Non-aversive handling and training techniques for laboratory animals are required to facilitate experimental and routine husbandry procedures, improving both animal welfare and scientific quality. Clicker training was utilized to develop training protocols for rabbits to refine stressful routine husbandry procedures usually associated with lifting (i.e., being picked up from the floor)/restraining (i.e., being held in the arms of a human) them. Thirteen female New Zealand White rabbits were trained over three weeks. All rabbits learned the predefined goal behaviors: they followed the target stick, jumped onto the weighing scale, entered a transport box, and reared while placing their front paws onto the trainer&#39;s hand. In addition, ten animals jumped from the floor onto the sitting trainer&#39;s lap and allowed the trainer to lift their paws off the surface while sitting on the trainer&#39;s lap. For some individuals, the protocols had to be adapted by additional interim steps. At the end of the training, the rabbits reliably showed the expected goal behaviors, even after short and long training breaks. With few exceptions, a familiar person other than the trainer could elicit the goal behaviors from the rabbits (generalization), though further sessions were required for generalization. In the voluntary approach test, the rabbits preferred interacting with the trainer in the 1st trial but spent as much time with an unfamiliar person as with the trainer in the 2nd trial. The behavioral observations suggested that picking the rabbits up with the transport box, as described in the protocol, instead of restraining them with the scruff of their neck and lifting them on the arm, was less aversive. All in all, the training protocols were feasible and can serve as a refinement strategy in laboratory animal facilities. In the interest of animal welfare, the training protocols should be applied wherever possible.

Author Spotlight: Training of Laboratory Animals for Gentle and Stress-Free Handling

Training protocols involving positive reinforcement for routine husbandry procedures in rabbits were developed and proved to be successful, as the rabbits reliably showed the trained behaviors at the weekly general examination. Picking the rabbits up with the transport box was less aversive than the conventional handling technique.

Non-aversive handling and training techniques for laboratory animals are required to facilitate experimental and routine husbandry procedures, improving ...

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a three host tick that requires a blood meal from a vertebrate host for each development stage, and the adult females require a blood meal for reproduction. Larval ticks attach to their host for 3 - 5 days for feeding and drop off the host when fully engorged. This dependency on several different hosts and the lengthy attachment time for engorgement complicates tick rearing in the laboratory setting. However, to understand tick biology and tick-pathogen interactions, the production of healthy, laboratory-reared ticks is essential. Here, we demonstrate a simple, cost-effective protocol for immature tick feeding on mice. We modified the existing protocols for decreased stress on mice and increased tick feeding success and survival by using disposable cages without mesh bottoms to avoid contact of ticks with water contaminated with mice urine and feces.

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

The production of healthy laboratory-reared ticks is essential to studies on tick biology, and tick-pathogen interactions. Here we demonstrate a simple protocol for immature tick feeding that is cost-effective and less stressful to mice.

Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a ...

Human Exposure to Larval Ticks for Tickborne Disease Research

A Model for Experimental Exposure of Humans to Larval Ixodes scapularis Ticks

Tickborne diseases are a significant public health problem in the United States and worldwide. Ticks are obligate blood-feeding arthropods; an ixodid tick must remain attached to the skin of the host and complete its multi-day feeding process to acquire its blood meal. Exposing animals to ticks is a common practice for studying host responses to tick bites and tickborne diseases. We developed the procedure, conducted the first human research study, and published the findings on exposing human volunteers to uninfected larval Ixodes scapularis ticks. This article describes the methodology used to construct the containment dressing, how to apply and secure the ticks to the host, how to maintain the dressing, and how to remove the ticks from the host. Exposing volunteers to tick bites is an experimental procedure and must be performed under a clinical research protocol approved by the appropriate regulatory authorities. This method allows for translational research to better understand the human response to tick bites and foster the development of diagnostics, prevention, and therapies for tickborne diseases.

Author Spotlight: Controlled Human Exposure Model for Tick Research and Lyme Disease Studies

This article presents the methodology for exposing humans to larval Ixodes scapularis for clinical research. The technique is relatively simple, tolerable by the research volunteers, and can be modified according to experimental needs. Such research involving human subjects must be conducted under clinical study protocols approved by the appropriate regulatory authorities.

Tickborne diseases are a significant public health problem in the United States and worldwide. Ticks are obligate blood-feeding arthropods; an ixodid tick ...

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Ticks are obligatory blood feeding parasites at all stages of development (except eggs) and are recognized as vectors of various pathogens. The use of mouse models in tick research is critical for understanding their biology and tick-host-pathogen interactions. Here we demonstrate a non-laborious technique for the feeding of immature stages of hard ticks on laboratory mice. The benefit of the method is its simplicity, short duration, and the ability to monitor or collect ticks at different time points of an experiment. In addition, the technique allows attachment of two individual capsules on the same mouse, which is beneficial for a variety of experiments where two different groups of ticks are required to feed on the same animal. The non-irritating and flexible capsule is made from easily accessible materials and minimizes the discomfort of the experimental animals. Furthermore, euthanasia is not necessary, mice recover completely after the experiment and are available for re-use.

In this study, a feeding system for nymphal and larval stages of hard tick was developed using a capsule attached to laboratory mouse. The feeding capsule is made from flexible materials and remains firmly attached to the mouse for at least one week and allows comfortable monitoring of tick feeding.

Ticks are obligatory blood feeding parasites at all stages of development (except eggs) and are recognized as vectors of various pathogens. The use of ...

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

Ticks are important ectoparasites that can vector multiple pathogens. The salivary glands of ticks are essential for feeding as their saliva contains many effectors with pharmaceutical properties that can diminish host immune responses and enhance pathogen transmission. One group of such effectors are microRNAs (miRNAs). miRNAs are short non-coding sequences that regulate host gene expression at the tick-host interface and within the organs of the tick. These small RNAs are transported in the tick saliva via extracellular vesicles (EVs), which serve inter-and intracellular communication. Vesicles containing miRNAs have been identified in the saliva of ticks. However, little is known about the roles and profiles of the miRNAs in tick salivary vesicles and glands. Furthermore, the study of vesicles and miRNAs in tick saliva requires tedious procedures to collect tick saliva. This protocol aims to develop and validate a method for isolating miRNAs from purified extracellular vesicles produced by ex vivo organ cultures. The materials and methodology needed to extract miRNAs from extracellular vesicles and tick salivary glands are described herein.

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

The present protocol describes the isolation of microRNAs from tick salivary glands and purified extracellular vesicles. This is a universal procedure that combines commonly used reagents and supplies. The method also allows the use of a small number of ticks, resulting in quality microRNAs that can be readily sequenced.

Ticks are important ectoparasites that can vector multiple pathogens. The salivary glands of ticks are essential for feeding as their saliva contains many ...

Tick Artificial Membrane Feeding for Ixodes scapularis

Ticks and their associated diseases are an important topic of study due to their public health and veterinary burden. However, the feeding requirements of ticks during both study and rearing can limit experimental questions or the ability of labs to research ticks and their associated pathogens. An artificial membrane feeding system can reduce these problems and open up new avenues of research that may not have been possible with traditional animal feeding systems. This study describes an artificial membrane feeding system that has been refined for feeding and engorgement success for all Ixodes scapularis life stages. Moreover, the artificial membrane feeding system described in this study can be modified for use with other tick species through simple refinement of the desired membrane thickness. The benefits of an artificial membrane feeding system are counterbalanced by the labor intensiveness of the system, the additional environmental factors that may impact feeding success, and the need to refine the technique for each new species and life stage of ticks.

Tick Artificial Membrane Feeding for Ixodes scapularis

Presented here is a method to blood feed ticks in vitro via an artificial membrane system to allow for partial or full engorgement of a variety of tick life stages.

Ticks and their associated diseases are an important topic of study due to their public health and veterinary burden. However, the feeding requirements of ...

A Versatile Model of Hard Tick Infestation on Laboratory Rabbits

Summary

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Chapters in this video

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Tickling, a Technique for Inducing Positive Affect When Handling Rats

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Training Laboratory Rabbits to Refine Routine Husbandry Procedures

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

A Model for Experimental Exposure of Humans to Larval Ixodes scapularis Ticks

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles

Tick Artificial Membrane Feeding for Ixodes scapularis