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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

DOI :

10.3791/58351-v

January 7th, 2019

January 7th, 2019

7,556 Views

1Department of Microbial Infection and Immunity, The Ohio State University, 2Department of Microbiology, The Ohio State University, 3Infectious Diseases Institute, The Ohio State University, 4Division of Biostatistics, College of Public Health, The Ohio State University

Here we describe a high-throughput fluorescence-based assay that measures the plasma membrane resealing efficiency through fluorometric and imaging analyses in living cells. This assay can be used for screening drugs or target genes that regulate plasma membrane resealing in mammalian cells.

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