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Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes

DOI :

10.3791/58116-v

October 4th, 2018

October 4th, 2018

8,451 Views

1Groupe de Recherche sur le Système Nerveux Central, and Département de Neurosciences, Université de Montréal, 2Faculté de Médecine Dentaire, Université de Montréal

Here we present a protocol to assess the organization of astrocytic networks. The described method minimizes bias to provide descriptive measures of these networks such as cell count, size, area, and position within a nucleus. Anisotropy is assessed with a vectorial analysis.

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