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Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

DOI :

10.3791/58034-v

August 16th, 2018

August 16th, 2018

11,953 Views

1Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute

In this protocol, we aim to describe a reproducible method for combining dissociated human pluripotent stem cell derived neurons and astrocytes together into 3D sphere cocultures, maintaining these spheres in free floating conditions, and subsequently measuring synaptic circuit activity of the spheres with immunoanalysis and multielectrode array recordings.

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Synaptic Microcircuit Modeling

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