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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

DOI :

10.3791/58162-v

10:14 min

November 1st, 2018

November 1st, 2018

12,906 Views

1Division of Renal-Electrolyte, Department of Medicine, University of Pittsburgh, 2Department of Cell Biology, University of Pittsburgh

The main focus of this protocol is to efficiently isolate viable primary glomeruli cultures with minimal contaminants for use in a variety of downstream applications. The isolated glomeruli retain structural relationships between component cell types and can be cultured ex vivo for a short time.

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Keywords Glomeruli

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