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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

DOI :

10.3791/58542-v

January 18th, 2019

January 18th, 2019

8,688 Views

1Department of Nephrology, Medical Faculty, Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

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Isolation

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