Aortic Root Sectioning and Oil Red O Neutral Fat Staining
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Results: Evaluation of the Aortic Root Phenotype in Aldosterone-induced Atherosclerosis in Apolipoprotein-E Knockout (ApoE -/-) Mice
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Conclusion
Transcript
This method can help answer key questions in the pathophysiology of early atherosclerosis in mice. The main advantage of this technique is the efficiency, rapidity with which aldosterone induces the development of atherosclerosis plaques in adult
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We describe a protocol to induce atherosclerosis in the aortic root of ApoE-/- mice fed with an atherogenic diet, through a continuous release of aldosterone. Methods to characterize plaque composition are also described.