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High-throughput Gene Tagging in Trypanosoma brucei

DOI :

10.3791/54342-v

11:26 min

August 12th, 2016

August 12th, 2016

7,571 Views

1Sir William Dunn School of Pathology, University of Oxford

Addition of a tag to a protein is a powerful way of gaining insight into its function. Here, we describe a protocol to endogenously tag hundreds of Trypanosoma brucei proteins in parallel such that genome scale tagging is achievable.

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High throughput Gene Tagging

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